CHEMICAL

AMOUNT (grams)

SIGMA NUMBER

cost $ US

amount purchased grams

setup # per chemical order

cost per liter CbMM

adenosine-3'(2')-phosphoric acid*H2O

0.3652

100233 (ICN)

5.10

1

3

1.863

beta-sitosterol

0.25

S5753

84.00

500

2000

0.042

biotin

0.015

B4501

9.70

100

6667

0.001

CaCl₂*2H2O

0.2205

C5080

31.55

500

2268

0.014

canocobalamine (vitamin b-12)

0.015

V6629

14.00

0.1

7

2.10

choline dihydrogen citrate

0.0885

C204

5.55

100

1130

0.005

Citric Acid*H₂O

0.6303

C7129

11.35

500

793

0.014

CuCl₂*2H₂O

0.0065

C6641

24.8

500

76923

0.001

cytidine-3'(2')-phosphoric acid

0.3232

101466(ICN)

15.55

1

3

5.026

cytochrome c

0.05

C2506

81.45

0.25

5

16.29

d-glucose

32.5

G7528

19.05

1000

31

0.619

dl-thioctic acid (lipoic acid)

0.015

T5625

11.00

1

67

0.165

Fe(NH4) ₂(SO₄) ₂*6H2O

0.0588

F3754

18.75

500

8503

0.002

glutathione(reduced)

0.204

G4251

36.05

10

49

0.735

glycine

0.722

G7126

17.45

500

693

0.025

guanosine-3'(2')-PO4(Na) ₂*H₂O

0.3632

101909(ICN)

19.15

1

3

6.955

K₃Citrate*H₂O

0.486

C8385

16.05

500

1029

0.016

KH₂PO₄

1.2255

P5379

23.95

500

408

0.059

KOH- 10%

10

P5958

13.90

250

25

0.556

L-alanine

1.395

A5824

26.40

25

18

1.47

L-argenine

0.975

A5006

6.50

25

26

0.254

L-aspartic acid

1.62

A9256

10.05

100

62

0.163

L-cysteine*HCl*H₂O

0.028

C7880

30.00

100

3571

0.008

L-glutamate(Na) *H₂O

0.55

G1626

10.35

100

182

0.057

L-glutamine

1.463

G3126

14.45

25

17

0.846

L-histidine

0.283

G8000

14.00

25

88

0.158

L-isoleucine

0.861

I2752

12.20

10

12

1.050

L-leucine

1.439

L800

10.30

25

17

0.593

L-lysine*HCl

1.283

L6027

19.15

100

78

0.246

L-methionine

0.389

M9625

10.50

25

64

0.163

L-phenyalanine

0.803

P2126

10.70

25

31

0.344

L-proline

0.653

P0380

11.85

25

38

0.310

L-serine

0.788

S4500

37.85

100

127

0.298

L-threonine

0.717

T8625

13.50

10

14

0.968

L-tryptophan

0.184

T0254

20.80

25

136

0.153

L-tyrosine

0.272

T3754

25.95

100

368

0.071

L-valine

1.02

V0500

14.50

25

25

0.592

Mg(OH) ₂

0.174

M5421

27.00

250

1437

0.019

MnCl₂*4H₂O

0.0222

M3634

27.75

500

22523

0.001

myo-inositol

0.0645

I5125

13.90

50

775

0.018

n-Acetylglucosamine

0.06

A8625

7.40

25

417

0.018

niacin (nicotinic acid)

0.03

N4126

8.45

100

3333

0.003

niacinamide

0.03

N3376

7.05

100

3333

0.002

p-aminobenzoic acid

0.03

A0129

19.05

100

3333

0.006

pantethine

0.015

P2125

20.15

1

67

0.302

pantothenate (Ca) pantothenic acid hemicalcium salt

0.03

P2250

9.30

25

833

0.011

pteroylglutamic acid (folic acid)

0.03

F7876

14.60

10

333

0.044

Pyridoxal*PO₄

0.015

P9255

16.40

1

67

0.246

pyridoxamine*2HCl

0.015

P6280

11.70

10

667

0.018

pyridoxine*HCl

0.03

P9755

9.90

5

167

0.059

riboflavin-5'-PO4(Na) *2H2O Flavin mono-nucleotide

0.03

F2253

60.25

0.1

3

18.080

Thiamine HCl

0.03

T4625

7.45

10

333

0.022

thyamine

0.1261

T0376

11.10

10

79

0.140

triethanolamine (2,2',2"-Nitrilotriethanol) (TEA)

t1377

13.25

100 ml

87

0.152

Tween 80

6.25

p8074

14.95

100 ml

14

1.047

uridine-3'(2')-phosphoric acid

0.3242

103220

13.85

1

3

4.490

ZnCl₂

0.0102

Z4875

18.85

500

49020

0.0003

Nalgene filter 0.45 micron

1

097401b

54.00

12

12

4.50

Nalgene filter 0.22 micron

4

097401a

54.00

12

3

18.00

totals

Initial cost for all chemicals

$1075.90

Cost per liter CbMM

$89.40

Total

45

Estimated Cost @ $5.00 / Hour

$225

Total Cost of Labor and Media

$497.55